a forthcoming talk of the Halle Plant Science Colloquium series will take place
on Tuesday, 13 November at 17:00 (s.t.)
in lecture hall E.02, Theodor-Lieser-Strasse 9 (Heide Süd Campus).
Jos Schippers (RWTH Aachen University) will talk about
The moonlighting function of superoxide dismutase depends on a novel class of transcriptional co-activators
Superoxide dismutase (SOD) has been regarded for more than 50 years as an important, pivotal antioxidant enzyme. However, recent work in yeast and humans indicate that copper-zinc SOD affects transcription inside the nucleus. Here we show that superoxide dismutase 1 (CSD1) from Arabidopsis and rice is a moonlighting protein. Next to its common scavenging function we reveal that CSD1 acts as a transcriptional regulator in the nucleus. Through fluorescent recovery after photobleaching (FRAP) experiments we found that CSD1 translocates to the nucleus upon stress. In addition, we revealed that CSD1 is a DNA-binding protein that recognizes a specific DNA motif. Moreover, we are using DNA affinity purification sequencing (DAP-seq) to uncover the genome-wide binding landscape of CSD1 in Arabidopsis. Interestingly, inside the nucleus CSD1 interacts with a member of the so far poorly characterized HEAVY-METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN (HIPP) family. Our data demonstrate that HIPP proteins are a novel class of conserved transcriptional co-activators both to in Arabidopsis and rice. The rapid translocation and transcriptional complex formation with HIPP enables CSD1 to be an important early player in a so far unknown redox-sensing mechanism. Exploring and understanding the moonlighting features of CSD1 will have major impacts on fundamental biology and potentially results in novel breeding strategies for crops.
For more information see:
If you would like to meet Jos Schippers personally, please contact Klaus Humbeck (Klaus.Humbeck@pflanzenphys.uni-halle.de).